A barrier to cost-effective AAV manufacturing in HEK293 cells is the poor biological understanding of the underlying pathways orchestrating AAV production and packaging, particularly in bioreactors. Limited analytical characterisation and investigation of cellular pathways involved in the AAV bioprocess hinders the development of novel, efficient production systems.
To close this knowledge gap, the Cell and Gene Therapy Catapult has conducted an unprecedented multi-omics study to unravel the biology of a scalable, transient transfection AAV process.
Here we demonstrate findings of the initial bioinformatics analysis, which has revealed unique molecular signatures and pathways kinetics differing between the high- vs. low producer clones, and how multi-omics integration has also proven useful for identifying novel features beyond those captured by single omics methods.
