The tool performs best with high-quality images:
- Even illumination across the image
- Image in focus
- Adequate exposure
Issues which may affect the image quality and therefore the confluency assessment, include:
- Debris, bubbles, scratches, etc
- Uneven illumination across the image
- Low resolution
- Condensation on the cell culture vessel
While we have done our best to develop a tool that is robust to these issues, the tool will be less accurate with poor quality images.
The tool assesses cell culture images magnified in the range x4 to x10. The tool can be used outside of this range, but the confluency estimate may be less accurate if the area contained within the image only represents a small area of the cell culture surface (high magnification). In such a scenario, we recommend taking multiple images for assessment to increase the reliability of the measurement. Any feedback on your experience with this tool, including issues with particular images, cell types, or imaging systems, is highly appreciated. This information will be used to further develop the tool.