Principles and Applications of Flow Cytometry for Therapeutics

This one-day intermediate course combines lectures with hands-on laboratory sessions to advance participants’ knowledge of flow cytometry. Using the Miltenyi MACSQuant X flow cytometer, delegates will explore exemplar experiments on compensation and immunophenotyping, guided by expert trainers with extensive experience across research and therapeutic manufacturing applications.

Learning objectives:

• Recall core principles of flow cytometry (fluidics, electronics, optics).
• Understand fluorochrome-conjugated antibodies for the identification and characterisation of cell sub-populations and the role of compensation.
• Explore experimental design: panel design, fixatives, staining, and blocking.
• Operate and maintain flow cytometry equipment using best practices.
• Understand immunophenotyping in advanced therapy contexts.
• Perform data analysis with FlowJo™ software.

Who should attend:

Scientists in advanced therapies and biomanufacturing (analytical development, process development, QC), as well as academic researchers in immunology, virology, molecular biology, and cancer biology. Also suitable for anyone seeking to build on foundational flow cytometry knowledge for therapeutic applications.
Concessions available for NHS staff, UK academics, and job seekers in flow cytometry for therapeutics.