Principles and Applications of qPCR

This two-day course will provide an introduction to real-time quantitative PCR (qPCR). Delegates will be guided by our expert trainers through a theoretical understanding and hands-on experience of the applications of qPCR, relevant for professionals within advanced therapies, biopharmaceuticals and academia.

Learning objectives:

• Understand laboratory health and safety pertaining to PCR workflows.
• Experience testing mock patient samples for SARS-CoV-2 .
• Gain competence in RNA extraction using automated Promega Maxwell kits.
• Gain competence in measurement of quality and quantity of RNA on NanoDrop spectrophotometer.
• Understand RNA degradation and be able to troubleshoot RNA extraction methods.
• Gain competence in setting up and running of QuantStudio 7 Flex Real-Time PCR System
• Understand qPCR Data analysis: data interpretation, normalisation, and statistical analysis.

Who should attend:

Individuals new to working in the advanced therapies and biomanufacturing sectors in roles such as analytical development, process development or quality control. Personnel within Molecular Biology, Immunology or employed as Field Applications Specialists seeking refreshers on laboratory skills for Continued Professional Development (CPD). Sales representatives and Business Development Managers wishing to gain a better understanding of qPCR with hands-on lab skills.